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Trial location: Marcos Juárez (Córdoba)
EEA INTA Trial carried out within the INTA-BIAGRO Technological
Agreement.
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Professionals
in charge:
Ing. Carlos Galarza (EEA INTA) Ing. Alejandro Perticari,
Ing.Juan C. Pacheco Basurco. (IMYZA-INTA-Castelar). Ing.
Roberto Echegaray (Biagro).
Special collaboration in the studies related to the occupation
of strains introduced in nodules: Dr. María Florencia
Del Papa, Dr. Antonio Lagares. (IBBM UNLP).
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History
and characteristics of the plot:
Previous
crops: Fescue for 10 years. From 1993 up to date,
direct drilling of first-rate soybean. Population of specific
soybean-nodulating bacteria: > 1,000 bac/g. of soil (NMP).
Soil
characterisation: Compound samples were taken and
sent to the Soil Institute of the INTA Marcos Juárez.
Results:
OM = 2.8%. Nitrate: 11.3 ppm. Passim = 33 ppm. S-Sulphate
= 10.3 ppm. K = 707 ppm. Mg = 380 ppm. Ca = 1856 ppm.
Na = 35.2 ppm. Zn = 1005 ppm. Mn = 5.4 ppm. Cu = 1.71
ppm. Fe = 56 ppm. B = 0.2 ppm. pH = 5.9 The soil is from
the Marcos Juárez Series, with fine loamy texture, well
aggregated soil structure and class 1 agricultural potential.
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Sowing
method:
direct
drilling.
Seed:
GM 4. VC Don Mario 4800 RR.
Planting
date:
November
27th, 2000. Inoculating method: INTA-BIAGRO preinoculation
(Nodulest inoculant + Biagro S1 sticker + Biagro S2 additive).
Weed
and plague control:
Winter, 2000 3 l/ha Glyphosate October, 2000 2.5 l/ha
Glyphosate + 100 cm3/ha cypermetrine. Preemergency Fipronil
(CLAP) 5 g.of pa/ha (ant and mole cricket control). Postemergency
2.7 Kg/ha of Roundup max.
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Experimental
design:
Randomised blocks. Six (6) repetitions in plots 6.6 yards long
and 4 rows with less than 4 inches of row spacing. Plots and
blocks separated by less than 40 inches.
Nodule
observation:
8 plants, including R2 and R3, were taken from each plot (See
comments). For the analysis of the percentage of occupation
in the nodules of the strains introduced by the inoculant, samples
of 8 nodules of the main root were taken from each plant (about
64 nodules per plot). Around 300-400 nodules were processed
for treatment and sent to the IBBM-UNLP.
Strains used:
The strains used for the trial from the IMYZA-INTA-Castelar
collection were specially prepared by Laboratorios Biagro S.A.
and formulated following the protocol for Nodulest 10 inoculant
to be used with the INTA-BIAGRO preinoculation technology. Strains:
E109, S5079, S5080, Tucumán 1, Salta 1.
Treatments:
1-Uninoculated
2-Uninoculated and fertilised with 400 N units (divided
in 5 applications of 80 UN: planting, V2, V4, R1, R3)
3, 4- E109 single and double dose.
5, 6- Tucumán 1 single and double dose.
7, 8- Salta 1 single and double dose.
9, 10- S5079 single and double dose.
11, 12- S5080 single and double dose. |
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Rainfalls:
November, 2000: 171.8mm. December, 2000: 100.9mm. January, 2001:
149.5mm. February, 2001: 125mm. March, 2001: 266mm. April, 2001:
76mm. until harvest. Harvest date: April 26th, 2001.
Harvest:
26 linear feet of row in each plot. Cultivable surface = 18
ft2. Density = 4 plants/linear feet.
Comments:
due to germination failure, the spaces without plant were re-sown
on December 16th, 2000 using the preinoculated seed prepared
for all the previous treatments (19 days after planting).
Summary
chart with up-to-date analysed data (the data is expressed as
an average for each strain):
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The
bar chart describes the level of response for the preinoculated
treatments against uninoculated ones in a soil with a
naturalised population of specific soybean-nodulating
bacteria that will be identified and characterised at
the IBBM.
Marcos
Juárez 2000/2001: Nodulest 10 INTA-BIAGRO Preinoculation
Strains
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(The
yellow bars show the response as a difference in Kg/ha against
the uninoculated control.)
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