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Trial location: Oncativo (Córdoba). IPEA.
Trial carried out within the INTA-BIAGRO Technological Agreement.
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Professionals
in charge:
Ing.
Jorge O. Petrello, Ing. Alcides Lamas, Ing. Jaime Noel.
(IPEA). Ing. Alejandro Perticari, Ing. Juan C. Pacheco
Basurco. (IMYZA INTA Castelar).
Ing. Roberto Echegaray (Biagro).
Colaboración especial para la realización de estudios
de ocupación de cepas introducidas en nódulos: Dra.
María Florencia Del Papa, Dr. Antonio Lagares. (IBBM
UNLP). Agradecimiento a los alumnos del IPEA.
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History
and characteristics of the plot:
Previous
crops: inoculated soybean. Alfalfa 1996- I 998. Peanut
I 998-1999. Sorghum 1999-2000. Population of specific
soybean-nodulation bacteria: > 1,000 bac/g. of soil
(NMP).
Soil
characterisation:
Compound
samples were taken and sent to the Soil Institute of
the INTA Castelar.
Results: OM = 2.17%. CO: 1.26%. NO = 0.14%. C/N = 9.
P=27.3 PPM. CE = 0.33 Ms/cm. pH = 6.1. The soil is from
the Oncativo Series, formed over loamy material of flat
hillocks and complex drainage. The soil is an aplustol
entic with fine loamy and sandy texture with poor developed
horizons. Related to IIIc class.
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Sowing
method:
direct drilling Seed: GM 5. VC Nidera 5436. Planting
date: November 15th, 2000
Inoculation
method:
INTA-BIAGRO preinoculation
(Nodulest 10 inoculant + Biagro S1 adhesive + Biagro
S2 additive).
Weed
and plague control:
10-08-00 1,7 l/ha Roundup full + 3 g/ha p.a. metasulfurón.
10-10-00 2,35 l/ha Clifosato + 115 cm3/ha cipermetrina.
31-10-00 3 l/ha Glifosato + 250 cm3/ha cipermetrina.
01-12-00 2,5 l/ha + 100 cm3/ha cipermetrina.
29- 12-00 2,5 l/ha + 80 cm3/ha cipermetrina.
08-02-01 2,5 l/ha + 100 cm3/ha cipermetrina.
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Experimental
design:
Randomised blocks. Six (6) repetitions in plots 6.6 yards long
and 4 rows with 20 inches of row spacing. Plots and blocks separated
by less than 40 inches.
Nodule observation:
8 plants in full bloom (R2) were taken from each plot. For the
analysis of the percentage of occupation in the nodules of the
strains introduced by the inoculant, samples of 8 nodules in
the main root were taken from each plant (about 64 nodules per
plot). Around 300-400 nodules were processed for treatment and
sent to the IBBM-UNLP.
Strains
used:
The strains used for the trial from the IMYZA-INTA-Castelar
collection were specially prepared by Laboratorios Biagro S.A.
and formulated following the protocol for Nodulest 10 inoculant
to be used with the INTA-BIAGRO preinoculation technology. Strains:
E109, S5079, S5080, Tucumán 1, Salta 1.
Treatments:
1- Uninoculated
2- Uninoculated and fertilised with 400 N units (divided
in 5 applications of 80 UN: planting, V2, V4, R1, R3)
3, 4- E109 single and double dose.
5, 6- Tucumán 1 single and double dose.
7, 8- Salta 1 single and double dose.
9, 10- S5079 single and double dose.
11, 12- S5080 single and double dose. |
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Rainfalls:
November, 2000: 23mm. December, 2000: 97mm. January,
2001: 153mm. February, 2001: 29.1mm. March, 2001: 145mm.
April, 2001: 68mm. until harvest.
Harvest date: April 25th, 2001. Harvest: 26 linear
feet of row in each plot. Cultivable surface = 13.5
ft2. Density = 4 plants/linear feet.
Comments: when the crop was developing flower
clusters, it withstood a heavy hailstorm with severe
defoliation, branch loss and injuries on the stem.
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Summary
chart with up-to-date analysed data (the data is expressed as
an average for each strain):
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The
bar chart describes the level of response for the preinoculated
treatments against uninoculated ones in a soil with
a naturalised population of specific soybean nodulating
bacteria that will be identified and characterised at
the IBBM.
Oncativo
2000/2001: Nodulest 10
INTA-BIAGRO Preinoculation Strains
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