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Trial location: Carabelas (Buenos Aires) Estancia La Serapia.
Owner: Mr Carlos Acuña
Trial carried out within the INTA-BIAGRO Technological Agreement.
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Professionals
in charge:
Ing.
A. Ladelfa, Ing. R. Amichetti (Cooperativa Agr. Ltda.
de Carabelas) Ing. Alejandro Perticari, Ing. Juan C. Pacheco
Basurco (IMYZA-INTA-Castelar) Ing. Roberto Echegaray (Biagro)
Special collaboration in the studies related to the occupation
of strains introduced in nodules: Dr. María Florencia
Del Papa, Dr. Antonio Lagares. (IBBM UNLP).
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History
and characteristics of the plot:
Previous
crops:
1997
campaign, corn. 1997-1998 Corn fertilised at sowing time
with P and N. 1998-1999 Soya. 1999-2000 Soya. Population
of specific bacteria soybean nodulation: > 100,000 bac/g.
of soil (NMP).
Soil
characterisation:
Compound
samples were taken and sent to the Soil Institute of the
INTA Castelar. Results: OM = 3%. CO = 1.74%. NO = 0.16%.
C/N = 10.9 Passim = 12.3 ppm. CE = 0.14 mS/cm pH = 6.3
The soil is a typical argiudol, of the Carabelas series,
class 1, of good depth and moderately well drained.
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Sowing
method:
Conventional tillage. (sequence: August, 2000 disk, harrow
and roller. Late December, 2000 the sequence is repeated.)
Seed:
GM 4. VC Maravilla 45 RR. (Relmó)
Planting
date:
January 8th, 2001. Inoculating method: INTA-BIAGRO preinoculation
(Nodulest 10 inoculant + Biagro S1 adhesive + Biagro S2
additive).
Weed
and plague control:
August 15th, 2000 3 l/ha Glyphosate. February, 2001 4
l/ha Roundup.
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Experimental
design:
Randomised
blocks. Six (6) repetitions in plots 6.6 yards long and 4 rows
with 20 inches of row spacing. Plots and blocks separated by
less than 40 inches.
Nodule
observation: 8 plants R3 were taken from each plot (See
comments). For the analysis of the percentage of occupation
in the nodules of the strains introduced by the inoculant, samples
of 8 nodules of the main root were taken from each plant (about
64 nodules per plot). Around 300-400 nodules were processed
for treatment and sent to the IBBM-UNLP.
Strains
used: The strains used for the trial from the IMYZA-INTA-Castelar
collection were specially prepared by Laboratorios Biagro S.A.
and formulated following the protocol for Nodulest 10 inoculant
to be used with the INTA-BIAGRO preinoculation technology. Strains:
E109, S5079, S5080, Tucumán 1, Salta 1.
Treatments:
1- Uninoculated
2- Uninoculated and fertilised with 160 N units (divided in
2 applications of 80 UN: planting, V4)
3, 4- E109 single and double dose.
5, 6- Tucumán 1 single and double dose.
7, 8- Salta 1 single and double dose.
9, 10- S5079 single and double dose.
11, 12- S5080 single and double dose. Rainfalls: January, 2001:
197mm. February, 2001: 33mm. March, 2001: 311mm. April, 2001:
116mm. May, 2001: 0mm. until harvest.
Harvest date: May 23rd, 2001.
Harvest: 26 linear feet of row in each plot. Cultivable
surface = 13.5 ft2. Density = 6 plants/linear feet.
Comments:
the preinoculation was done in the shade and the sowing was
done with a room temperature of 102ºF and a wind-chill factor
above 104ºF. Before sowing, the rows were irrigated with an
equivalent of 30mm.
Summary
chart with up-to-date analysed data (the data is expressed as
an average for each strain):
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The
bar chart describes the level of response for the preinoculated
treatments against uninoculated ones in a soil with a
naturalised population of specific soybean nodulating
bacteria that will be identified and characterised at
the IBBM.
Carabelas
2000/2001: Nodulest 10 INTA-BIAGRO Preinoculation Strains
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